irradiation cell use


Irradiation - Wikipedia


Ion irradiation is routinely used to implant impurities atoms into materials, especially semiconductors, to modify their properties.This process, usually known as ion implantation, is an important step in the manufacture of silicon integrated circuits.. Ion irradiation. Ion irradiation means in general using particle accelerators to shoot energetic ions on a material.

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How could irradiated splenocytes be APCs? - ResearchGate


In CD4 T cell activation, we used to stimulate CD4T cells with CD3 agonist antibodies + splenocytes in vitro culture condition. I don't know how the irradiated splenocytes could play a role as APCs.

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IRRADIATION PROTOCOL - Geisel School of Medicine


irradiation time. Specify mouse identity, number, dose and use of a Lucite pie. Tag mice prior to irradiation (several days in advance is best). After irradiation, cells can be injected immediately or up to 2-3 days later. Animals go into a barrier room. For the first 2-4 weeks after the irradiation day, supplement the drinking water with ...

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Non‐thermal cytocidal effect of infrared irradiation on ...


May 19, 2010· As infrared penetrates the skin, thermal effects of infrared irradiation on cancer cells have been investigated in the field of hyperthermia. We evaluated non‐thermal effects of infrared irradiation using a specialized device (1100–18000 nm with filtering of wavelengths between 1400 and 1500 nm and contact cooling) on cancer cells.

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Alternative Splicing of SMAD4 and Its Function in HaCaT ...


Cell physiology and behaviour was progressively examined with the help of an inverted microscope. UVB Irradiation. HaCaT cells were subcultured for 48 hours (80% confluence) in 60mm cell culture dishes in standard medium. UVB irradiation was carried out by using two 8-watts UVB lamps (SANKEY, Japan).

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Determination of cell survival after irradiation via ...


For studying proliferation and determination of survival of cancer cells after irradiation, the multiple MTT assay, based on the reduction of a yellow water soluble tetrazolium salt to a purple water insoluble formazan dye by living cells was modified from a single-point towards a proliferation assay. This assay can be performed with a large number of samples in short time using multi-well ...

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Irradiation: What It Is, What It Does, and How It Affects ...


"Irradiation is a technology that leaves no residue in food products and destroys the DNA of microorganisms to prevent them from multiplying. When you irradiate food, you produce an effect on microorganisms due to their effects on DNA and cell membranes. Photons, electrons, or x-rays strike the cell membranes and the DNA, damaging both.

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Development of a biosafety level-2 facility for ...


Here we present an ultra-low flux irradiation facility and associated fluence control for biological cell and tissue irradiation under sterile biosafety level 2 (BSL-2) conditions suitable for use with low energy (1-3.4 MeV) protons. The operation is demonstrated using murine astocytes.

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Gamma Irradiation Sterilization Process & Services ...


Jan 03, 2012· For studying proliferation and determination of survival of cancer cells after irradiation, the multiple MTT assay, based on the reduction of a yellow water soluble tetrazolium salt to a purple water insoluble formazan dye by living cells was modified from a single-point towards a proliferation assay. This assay can be performed with a large number of samples in short time using multi-well ...

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5 SELF-CONTAINED IRRADIATORS | Radiation Source Use and ...


Washington, DC: The National Academies Press. doi: 10.17226/11976. ... In some cases for cell irradiation, units with energy as low as 50 kVp are employed. These units provide dose rates of 0.5 to 1.5 Gy/min depending on the target-to-surface distance. Some of these units are still in use.

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Irradiation of Mammalian Cultured Cells with a Collimated ...


Funayama, T., Wada, S., Kobayashi, Y. and Watanabe, H. Irradiation of Mammalian Cultured Cells with a Collimated Heavy-Ion Microbeam. Radiat. Res. 163, 241–246 (2005). As the first step for the analysis of the biological effect of heavy charged-particle radiation, we established a method for the irradiation of individual cells with a heavy-ion microbeam apparatus at JAERI-Takasaki.

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Gamma Irradiation Systems | Nordion


Not only does Nordion provide Cobalt-60 to companies in the medical device industry, food industry and other industries for innovative applications for sterilization and irradiation of products, we also manufacture irradiation systems.

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Gamma Irradiation Sterilization Process & Services ...


Processing with gamma irradiation yields quick turnaround time, easily penetrating packaging and product, and is ideal for many types of materials. What is Gamma Irradiation? The radioisotope cobalt 60 is the energy source for use in gamma irradiation sterilization with the irradiation process taking place in a specially designed cell.

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Blood Transfusion : Irradiation of blood products


The Biological Irradiation Core (BIC) consists of two facilities. One is dedicated to tissue samples and the other for small animals. Each room contains one RS-2000 irradiator supplied by RAD Source. Cell Irradiation. The cell irradiator is located in BCC 5.12.369, Room 369 on the 5th floor of the Fred and Pamela Buffett Cancer Center.

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Total body irradiation: A practical review


Total body irradiation (TBI) with megavoltage photon beams is one component used in treating several diseases, including multiple myeloma, leukemias, lymphomas and some solid tumors. 1,2 In combination with chemotherapy, TBI is most commonly used as part of the conditioning regimen prior to hematopoietic stem cell transplantation. 1,3,4 TBI provides a uniform dose of radiation to the entire ...

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Targeted Irradiation of Mammalian Cells Using a Heavy-Ion ...


Furthermore, Funayama and coworkers report the irradiation of CHO cells using a collimated beam of argon ions from the Tiara accelerator (Japan) . In this case, the targeted nuclei were determined retrospectively by track etching. However, a targeted irradiation of single selected cells with heavy ions is desirable in most cases and still ...

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Effects of gamma irradiation on cell cycle, apoptosis and ...


Jul 03, 2013· Detection of apoptotic cells and analysis of the cell cycle. Following irradiation, the trypsinized cells were washed and collected by centrifugation. The cell numbers were counted using a hemocytometer. RNase (Sigma, St. Louis, MO, USA) and propidium iodide (Sigma) were added to the cells and mixed using a vortex.

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Expansion of cytotoxic natural killer cells using ...


Sep 11, 2017· We developed a new in vitro NK cell expansion method using a combination of the anti-CD16 mAb with irradiated autologous PBMCs without the use of cancer cells or other genetically modified feeder ...

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Irradiation of PBMCs- dose, freezing possible?


Using the PBMCs as "feeder cells" 50 Gy OR 5000 rad is sufficient, Seda. I typically irradiate my feeder cells with 50 Gy, (if you can let them rest overnight) remember to wash them and resuspend ...

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Using human embryonic stem cells to treat radiation ...


In addition, many of these side effects pose particular threats to pediatric patients undergoing radiotherapy, since children contain more stem cells and suffer higher absolute losses of these cells after irradiation.Based on the foregoing, we will explore the potential utility and risks associated with using human embryonic stem cells (hESC ...

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Focus Application - ACEA Biosciences | The Cell Analysis ...


Since cell death begins only 48 hours after irradiation, cells presumably undergo apoptosis, however, further analysis is needed to investigate the mode of cell death in more detail. Taken together, the continuous online monitoring of HEK-293 cells using the xCELLigence System revealed detailed information about the cellular

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Cell Research Irradiator - Rad Source Technologies


The RS 1800 Biological Research Irradiator. The excellent irradiator for the irradiation of cells. This unit, in use throughout the world, uses the same technology as the Rad Source RS 3000 blood irradiator which is considered by the FDA to be "Substantially equivalent" to Cesium-137 gamma irradiators.

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The use of gamma-irradiation and ultraviolet-irradiation ...


The use of gamma-irradiation and ultraviolet-irradiation in the preparation of human melanoma cells for use in autologous whole-cell vaccines Donna H Deacon, 1, 2 Kevin T Hogan, 1, 2 Erin M Swanson, 1 Kimberly A Chianese-Bullock, 1, 2 Chadrick E Denlinger, 1 Andrea R Czarkowski, 1 Randy S Schrecengost, 1 James W Patterson, 3 Mark W ...

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Update on the Use of Irradiated Blood Components for ...


Dec 14, 2018· Less clear are other patients with cancer, especially leukemia's and lymphomas. Unresolved questions seem to be whether irradiated blood is ever indicated for solid organ transplant patients, and when exactly to start and possibly stop use of irradiated blood in hematopoietic stem cell transplant (HSCT) patients who successfully engraft.

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Sterilization by Gamma Irradiation | Tuttnauer


Another common application of sterilization by Gamma irradiation is food. Food sterilization by gamma irradiation is the process of exposing food to ionizing radiation to destroy microorganisms, namely bacteria, or insects that might be present in the food. Advantages

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Irradiation - Blood bank


Irradiation: Why are irradiated cellular blood components needed? Irradiated blood is given to prevent rare complications of transfusion called transfusion- associated graft-versus-host disease (TA-GvHD). What TA-GvHD? TA-GvHD is a rare but serious complication of blood transfusion caused by white blood cells (lymphocytes) in the blood transfused.

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Gamma Irradiation of Blood Products (7/22/93) Date: All ...


irradiation.1,25,26 White cell filters have not been effective to date, but their use continues under investigation. B. IRRADIATION OF BLOOD PRODUCTS TO PREVENT GVHD.

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The Facts about Food Irradiation


Food irradiation is a promising new food safety technology that can eliminate disease-causing microorganisms such as E. coli O157:H7, Campylobacter, and Salmonella from foods. The Food and Drug Administration has approved irradiation of meat and poultry and allows its use for a variety of other foods, including fresh fruits and vegetables, and ...

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Part 4: Irradiation Calculations | ITACA


The measured irradiation data that can be referenced is normally in the form of the monthly average daily (MAD) irradiation on a horizontal plane; that is the total irradiation measured in each month divided by the number of days in the month. This figure is made up of the beam and diffused components described in section 2.5 and it is assumed ...

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Effects of Normothermic Conditioned Microwave Irradiation ...


Microwave irradiation (described as 'MW') was applied for 1 h with the temperature of the cultured cells maintained at 37 °C, whereas the temperature inside the applicator was set at 10 °C.

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